Improving the Toolbox of Bioreductions through the use of Continuous Flow Systems
An exciting new paper from the University of Rio de Janeiro (UFRJ) uses the Syrris Asia System to demonstrate improvements to bioreductions.
Using the Syrris Asia Flow Chemistry System, researchers at the UFRJ have demonstrated an interesting alternative to the bioreduction of β-ketoesters by immobilized microorganisms.
The use of enzymes to facilitate enantiomerically pure compounds has been shown to provide good alternatives to chemical catalysts and continuous flow biocatalyzed reactions are being increasingly used by the chemistry community.
Bioreduction of ethyl 3-oxohexanoate by immobilized K. marxianus cells and tert-butyl 3-oxobutanoate immobilized Rhodotorula rubra cells
The immobilization of microorganisms by calcium alginate entrapment allows systems of this type to be more robust and readily re-used and recycled, a big advantage over using whole cells which cannot be recycled.
Using this methodology the group obtained β-hydroxyesters in excellent yields and high enantiometric excess (>99%). By varying the β-ketoester structure and immobilized microorganism the absolute stereochemistry could also be controlled.
Syrris Atlas Calorimeter benefits process scale-up
A Syrris Atlas Calorimeter is proving advantageous to the Kyushu Works Manufacturing Technology Department of Nippon Steel & Sumikin Chemical Co., Ltd. in Japan, aiding scale-up of new product processes.
Mr. Kenji Umeda from the Production & Technical Department explained: “Our department performs mass production studies for new product development, supporting the company’s business of manufacturing coal tar, basic and fine chemicals. During product development, we undertake a series of processes from small-scale laboratory studies through to large-scale production. To ensure safe practices, we need to acquire calorimetric data during process scale-up and, after looking at various products, chose the Atlas Calorimeter with optional Atlas Syringe Pump for its accuracy and ease of use.”
"We have had the Atlas Calorimeter for about a year now, and use it on a regular basis for new product development processes. The system was easy to implement in our laboratory and provides additional capabilities that have not previously been available, allowing us to perform multi-step calorimetry experiments and automatically calculate calorimetric data from the experimental results. The Atlas Calorimeter's accurate temperature control and reproducibility are particularly beneficial, helping to improve the precision of our studies. Syrris is very helpful – the company even translated the manual into Japanese – and always responds promptly to our requests."
Learn about the latest flow chemistry technologies and techniques, and get hands-on practical experience with our Asia Flow Chemistry Systems at our FREE 1-Day Flow Chemistry Workshops!
These free events, which combine theoretical discussion with hands-on sessions, enable chemists to develop their understanding of the benefits that flow chemistry can bring to their experiments, and learn about modern flow chemistry techniques which can make major improvements to the safety, quality and speed of chemistry.
We have received fantastic feedback from previous delegates working in a wide range of application areas. Join us for our next workshop near you!